hepg2 reporter gene cells  (InvivoGen)

Journal: Toxicology Reports

Article Title: Exploring the biological impact of bacteria-derived indole compounds on human cell health: Cytotoxicity and cell proliferation across six cell lines

doi: 10.1016/j.toxrep.2024.101883

Figure Lengend Snippet: Human cell lines used in this study (ATCC: American Type Culture Collection. EMEM: Eagle’s minimum essential medium. FBS: fetal bovine serum).

Article Snippet: HepG2-LUCIA™ , HepG2 reporter gene cells for AhR activity , InvivoGen , EMEM + 10 % FBS + 1X non-essential amino acids medium + 100 μg/mL Normocin + 100 μg/mL Zeocin , Gao et al., .

Techniques: Activity Assay

Journal: Toxicology Reports

Article Title: Exploring the biological impact of bacteria-derived indole compounds on human cell health: Cytotoxicity and cell proliferation across six cell lines

doi: 10.1016/j.toxrep.2024.101883

Figure Lengend Snippet: Cytotoxic evaluation (EC 50 ) in µM following 24 h exposures of several indole metabolites on different cell lines: enterocytes (Caco-2), hepatocarcinoma cells (HepG2), hepatocytes (HepaRG), lung fibroblasts (MRC-5), mesenchymal stem cells (MSC), and breast epithelial (T47D). Data are presented as average ± standard deviation (n = 8–16). IPA: Indole-3-propionic acid. IAA: Indole-3-acetic acid. I3CA: Indole-3-carboxylic acid. I3A: Indole-3-carboxaldehyde. 3-MI: 3-Methylindole.

Article Snippet: HepG2-LUCIA™ , HepG2 reporter gene cells for AhR activity , InvivoGen , EMEM + 10 % FBS + 1X non-essential amino acids medium + 100 μg/mL Normocin + 100 μg/mL Zeocin , Gao et al., .

Techniques: Standard Deviation

Journal: Toxicology Reports

Article Title: Exploring the biological impact of bacteria-derived indole compounds on human cell health: Cytotoxicity and cell proliferation across six cell lines

doi: 10.1016/j.toxrep.2024.101883

Figure Lengend Snippet: Aryl hydrocarbon receptor (AhR) activation by indole metabolites (IPA: indole-3-propionic acid; IAA: indole-3-acetic acid; I3CA: indole-3-carboxylic acid; I3A: indole-3-carbaldehyde; 3-MI: 3-methylindole; Indole: indole) using the HepG2-Lucia™ AhR gene-reporter cells. Data obtained from positive controls are also shown (TCDD: 2,3,7,8-tetrachlorodibenzo-p-dioxin; BaP: benzo[ a ]pyrene; BNF: β-naphthoflavone). Data are presented as EC 50 and average (95 % Confidence Interval) (n = 12–18).

Article Snippet: HepG2-LUCIA™ , HepG2 reporter gene cells for AhR activity , InvivoGen , EMEM + 10 % FBS + 1X non-essential amino acids medium + 100 μg/mL Normocin + 100 μg/mL Zeocin , Gao et al., .

Techniques: Activation Assay

Journal: The Journal of steroid biochemistry and molecular biology

Article Title: Plasticizers used in food-contact materials affect adipogenesis in 3T3-L1 cells

doi: 10.1016/j.jsbmb.2018.01.014

Figure Lengend Snippet: HepG2 cells were transfected with pcDNA3-PPARγ, DR1-Luc, and pCMV-β-galactosidase vectors, then were treated with scalar concentrations of Rosiglitazone or plasticizers as described under Material & Methods. Luciferase activities are reported as fold changes of luminescence of treated cells versus control (=1) ± SEM (n=3). * p<0.05.

Article Snippet: Transfection and reporter gene assays HepG2 human hepatoblastoma cell line (ATCC ® HB-8065TM; ATCC, USA) was used for gene reporter assays; cells were plated on a 24 well plate and then transfected with the following constructs [ 33 , 34 ]: (1) 1.5 μg DR1-Luc (containing a direct repeat 1 upstream of luciferase gene), (2) 100 ng pCMV-βgal (pCMV-β-galactosidase normalization plasmid), and (3) 400 ng pcDNA3-PPARγ (an expression vector for human PPARγ) using Lipofectin (Invitrogen).

Techniques: Transfection, Luciferase, Control